Application

This Milli-Mark Anti-CD15-FITC Antibody, clone C3D-1 is validated for use in FC for the detection of CD15.

General description

CD15 is a carbohydrate antigen carried by both glycoproteins and glycoliplds. The structure of the antigen was determined to be Galb1→4[Fuca1→3]GIcNACb1→3Galb1→R. CD15 serves as a ligand for selectins and might also be involved in cell adhesion through a direct Lewis X-Lewis X interaction. CD15 is expressed mainly on mature granulocytes and monocytes but also on immature bone marrow cells (Kannagi, 1997).
Anti-CD15, C3D-1, was included in the Sixth International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with CD15 (Kannagi, 1997).

Immunogen

Purified human neutrophils

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified Mouse monoclonal IgM conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

Activated Sepharose

Quality

Evaluated by flow cytometry using human PBMCs

Specificity

Antibody recognizes Human CD15 antigen.